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Education

Ph.D Candidate

2019

Ph.D Student

2018

Master of Science

2015

Bachelor of Science

2011

Professional Experience

* Research interest: Molecular Medicine, Molecular Biology, Medical Physiology, Oncology, Biotechnology, Cell Biology, Biochemistry, and Genetics.

* Medical and Molecular techniques and methods: o PCR: qPCR, RT-PCR, and conventional PCR, PCR optimization. o Immunoblotting: Western blotting, ELISA, dot-blot. o Immunoprecipitation: Chromatin immunoprecipitation (Chip-Seq and Chip-qPCR), RNA immunoprecipitation (RIP-seq and RIP-qPCR), Co-immunoprecipitation (Co-IP) followed by WB or Mass spectrometry, and Crosslinking immunoprecipitation followed by sequencing (CLIP-seq). o Immunostaining: Immunocytochemistry o Cell and Tissue culture: cell culture of adherent and suspension cells, cell passages, and cryopreservation. o Assays: Wound healing, migration, invasion, and proliferation assay. o Primer and Probe designing o Extraction methods: Nucleic acid extraction (Eukaryotic, prokaryotic, and gel) and protein extraction (Cytoplasmic and Nuclear) o Protein determination: BCA assay, Bradford assay, and Qubit 4 based determination) o Transfection (lipofectamine and electroporation) and transformation (heat shock). o Gene knockdown (RNA silencing) o Gene knock-in (plasmid insertion) o Site-directed mutagenesis (generate mutation) o Electrophoresis: PAGE, Agarose Gel Electrophoresis. o Staining gel and blots: Silver staining, Ponsue Red, BradFord, and Coomassie Blue. o Microscope: Confocal Microscope (nondependent), widefield fluorescence microscope, Light Microscope, Dark-field Microscopy, Dissecting Microscope. o Others: Sonication (Eukaryotic and Prokaryotic), Spectrophotometer, Nanodrop, Plate reader, Autoclaving, ...etc.

* Medical and Molecular software: o Creation of Image, Sketch and Diagram: Biorender, Adobe Photoshop, IBS, GraphPad prism, and Excel. o Statistical analysis: GraphPad prism o Sequencing analysis: KEGG, Venn graph, Reactome, and Heatmap. o Image analysis software: ImageLab (Western blot), ImageJ, GraphPad, and PhotoScape... etc. o Gene manipulation and Primer design software: Snap gene and Serial Cloner o PCR software: QuantStudio™ Design & Analysis Software (3, 5, and 6 pro) and Step One Software v2.3 o Confocal Microscope: ZEN o Quality and quantity detection software: Nanodrop and Plate reader software o Referencing software: Mendeley and RefWorks. o Excel: for Analyzing NGS data, qPCR fold enrichment, RIP-seq, Chip-seq fold enrichment.

* Medical and Biological Website: o Signaling pathway analysis: KEGG and Reactome o Alignment Tools: NCBI nucleic acid BLAST and protein BLAST o Oligo (primer and probe) designing tools and calculation for PCR, qPCR, cloning, and Sanger sequencing: Primer-BLAST, Primer3, ThermoCalc. and RC, OligoPerfect. o Molarity, Mass, OD, and dilution calculation: NEBio and GraphPad o RNA Structure Prediction: ViennaaRNA Web Services. o Protein-Protein interaction and connection: STRING o Bioinformatics: SMS, Venn graph, dosztanyi web, Phobius, Biobam, MPI, GOrilla, RCSB, PhosphositePlus, and IUpred. o Sketch and Diagram design website: Biorender

Academic Title

2017

Assist Lecturer

2017-01-01
2013

Assist Researcher

2013-03-25

Awards

2024

The role of EZH2 in TKS4-deficient colorectal carcinoma cells

2024-08
2023

A Beginner's guide to DNA sequencing

2023-10

The role of Ezh2 in the Tks4 deficient cells inducing migration, invasion, and EMT-related processes

2023-03
2022

A Beginner's Guide to DNA Sequencing Recent Advances

2022-10

Next generation sequencing and its Clinical Applications

2022-09

ncRNA-seq results explained: What you can explore with non-coding RNA data

2022-07

Next generation sequencing solutions for transcriptomics and epigenetics research

2022-05

A Beginner's guide to DNA-seq-Bioinformatic analysis

2022-04
2021

The role of Ezh2 and Tks4 in EMT-related processes

2021-11

The complete guide to research paper writing

2021-04
2015

Certificate of teaching methods

2015-12

Evaluation

2021

The role of long non-coding RNAs, TKS4 and EZH2 in the process of EMT

2021-06-29

The role of EZH2 and long non-coding RNAs in TKS4-deficient induced epithelial-mesenchymal transition-like process

2020-06-26

2020

The role of long non-coding RNAs, TKS4 and EZH2 in the process of EMT

2021-06-29

The role of EZH2 and long non-coding RNAs in TKS4-deficient induced epithelial-mesenchymal transition-like process

2020-06-26

Training Course

2015

Certificate of Teaching Methods

2015-09-15,2015-10-25

Thesis

2015

EXPRESSIONAL PROFILING OF LINC00663 RNA IN VARIOUS HUMAN TISSUES AND CANCER CELL LINES

2015-05-25

Published Journal Articles

2024

Morphological Changes Induced by TKS4 Deficiency Can Be Reversed by EZH2 Inhibition in Colorectal Carcinoma Cells

2024-04
2023

KMT2D preferentially binds mRNAs of the genes it regulates, suggesting a role in RNA processing

2023-12

Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells

2023-01
2016

A novel variable exonic region and differential expression of LINC00663 non-coding RNA in various cancer cell lines and normal human tissue samples

2016-01

Conference

2024

The role of EZH2 in TKS4-deficient colorectal carcinoma cells

2024-08

Investigating the role of RNA binding in the cellular function of KMT2D

2024-08
2023

The role of Ezh2 in the Tks4 deficient cells inducing migration, invasion, and EMT-related processes

2023-03
2021

The role of Ezh2 and Tks4 in EMT-related processes

2021-11

The RBR-polyQ Region Of KMT2D: Unique Sequence-Specific Function

2021-11
2015

Kanser hücrelerinde non-coding LINC00663 RNA’nın yeni splice varyantlarının belirlenmesi ve bu splice varyantlarınpotansiyel ikincil yapılarının gösterilmesi

2015-10
2014

Çeşitli İnsan Dokuları ve Hücre Hatlarında non-coding LINC00663 RNA’nın Gen İfade Profili

2014-09

Presentation

2024

The role of EZH2 in TKS4-deficient colorectal carcinoma cells

2024-08

The role of EZH2 in TKS4-deficient colorectal carcinoma cells

2024-08

Morphological changes induced by TKS4 deficiency can be reversed by EZH2 inhibition

2024-03
2023

The role of LncRNAs in the Tks4 deficient cells inducing migration, invasion, and EMT-related processes

2023-12

The role of EZH2 in the TKS4 deficient cells inducing migration, invasion, and EMT-related processes

2023-03
2022

Tks4 at the crossroads of signaling pathways

2022-09

The role of Ezh2 and Tks4 in EMT-related processes

2022-04

Absence of Scaffold Protein Tks4 Disrupts Several Signaling Pathways in Colon Cancer Cells

2022-02
2021

The role of EZH2 and TKS4 in EMT-related processes

2021-12
2020

Structural and functional characterization of the protein-RNA complexes in the epithelial-mesenchymal transition

2020-03

Workshop

2015

E-JOURNAL OF UNIVERSITY OF ZAKHO “A PEER REVIEW SYSTEM”

2015-09