Education
Professional Experience
* Research interest: Molecular Medicine, Molecular Biology, Medical Physiology, Oncology, Biotechnology, Cell Biology, Biochemistry, and Genetics.
* Medical and Molecular techniques and methods: o PCR: qPCR, RT-PCR, and conventional PCR, PCR optimization. o Immunoblotting: Western blotting, ELISA, dot-blot. o Immunoprecipitation: Chromatin immunoprecipitation (Chip-Seq and Chip-qPCR), RNA immunoprecipitation (RIP-seq and RIP-qPCR), Co-immunoprecipitation (Co-IP) followed by WB or Mass spectrometry, and Crosslinking immunoprecipitation followed by sequencing (CLIP-seq). o Immunostaining: Immunocytochemistry o Cell and Tissue culture: cell culture of adherent and suspension cells, cell passages, and cryopreservation. o Assays: Wound healing, migration, invasion, and proliferation assay. o Primer and Probe designing o Extraction methods: Nucleic acid extraction (Eukaryotic, prokaryotic, and gel) and protein extraction (Cytoplasmic and Nuclear) o Protein determination: BCA assay, Bradford assay, and Qubit 4 based determination) o Transfection (lipofectamine and electroporation) and transformation (heat shock). o Gene knockdown (RNA silencing) o Gene knock-in (plasmid insertion) o Site-directed mutagenesis (generate mutation) o Electrophoresis: PAGE, Agarose Gel Electrophoresis. o Staining gel and blots: Silver staining, Ponsue Red, BradFord, and Coomassie Blue. o Microscope: Confocal Microscope (nondependent), widefield fluorescence microscope, Light Microscope, Dark-field Microscopy, Dissecting Microscope. o Others: Sonication (Eukaryotic and Prokaryotic), Spectrophotometer, Nanodrop, Plate reader, Autoclaving, ...etc.
* Medical and Molecular software: o Creation of Image, Sketch and Diagram: Biorender, Adobe Photoshop, IBS, GraphPad prism, and Excel. o Statistical analysis: GraphPad prism o Sequencing analysis: KEGG, Venn graph, Reactome, and Heatmap. o Image analysis software: ImageLab (Western blot), ImageJ, GraphPad, and PhotoScape... etc. o Gene manipulation and Primer design software: Snap gene and Serial Cloner o PCR software: QuantStudio™ Design & Analysis Software (3, 5, and 6 pro) and Step One Software v2.3 o Confocal Microscope: ZEN o Quality and quantity detection software: Nanodrop and Plate reader software o Referencing software: Mendeley and RefWorks. o Excel: for Analyzing NGS data, qPCR fold enrichment, RIP-seq, Chip-seq fold enrichment.
* Medical and Biological Website: o Signaling pathway analysis: KEGG and Reactome o Alignment Tools: NCBI nucleic acid BLAST and protein BLAST o Oligo (primer and probe) designing tools and calculation for PCR, qPCR, cloning, and Sanger sequencing: Primer-BLAST, Primer3, ThermoCalc. and RC, OligoPerfect. o Molarity, Mass, OD, and dilution calculation: NEBio and GraphPad o RNA Structure Prediction: ViennaaRNA Web Services. o Protein-Protein interaction and connection: STRING o Bioinformatics: SMS, Venn graph, dosztanyi web, Phobius, Biobam, MPI, GOrilla, RCSB, PhosphositePlus, and IUpred. o Sketch and Diagram design website: Biorender
Academic Title
2017
2013
Awards
2024
2023
2022
2021
2015
Evaluation
2021
The role of long non-coding RNAs, TKS4 and EZH2 in the process of EMT
2021-06-29
The role of EZH2 and long non-coding RNAs in TKS4-deficient induced epithelial-mesenchymal transition-like process
2020-06-26
2020
The role of long non-coding RNAs, TKS4 and EZH2 in the process of EMT
2021-06-29
The role of EZH2 and long non-coding RNAs in TKS4-deficient induced epithelial-mesenchymal transition-like process
2020-06-26