The diverse genus Alternaria encompasses fungi with various lifestyles, including pathogenic, saprophytic, and endophytic species. Inspection of the endophytes of two oak plant species (Quercus aegilops and Q. infectoria) from the mountainous area of Duhok province of the Kurdistan region of Iraq resulted in the isolation of seven Alternaria species. These include A. alternata, A. angustiovoidea, A. consortiales, A. doliconidium, A. malorun, A. sorghi, and A. tenuissima. Of these, A. angustiovoidea, A. doliconidium, A. malorun, and A. sorghi were recorded for the first time in Iraq. The seven Alternaria species were also reported for the first time as endophytes on the two Quercus species. The identification of these strains was based on DNA sequencing information utilizing the ITS and LSU genetic markers and morphological characteristics, including details of colony growth, conidial characteristics, and reproductive structures. Furthermore, phylogenetic analysis was conducted to establish the evolutionary relationships among these Alternaria isolates. This investigation gives a basis for the understanding of the distribution of endophytic fungi associated with forest trees in the country.
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