PowerLab Data Acquisition System, Tissue-Organ Bath unit, Balance (Max 600 g ), Force Transducer ( 0 to 20 grs ), Thermo/Bath Circulator, Computer Running, Chart Software, Magnetic Mixer, Magnetic Stirrer, pH-Meter, Water Distillator, ML 2001/2, Pressure resistant Water Deionizer, SG 2000 SK. Ion exchange P2/30 with 3/4”, Molecular Devices: Nucleic acid and protein extraction , gel electrophoresis(agarose and Page ) Western bloting , transformation,transfection ,PCR:sqPCR , qPCR, RT-PCR and Multiplex PCR. Spectrophotometer.
A Comparative Study on the Effect of Hydrogen Sulfide on Isolated aortic smooth muscle contractility of Fishes, Pigeons and Rats
The current study targeted the investigation of the molecular mechanism of contractility induced hydrogen sulfide (H2S) in fish, pigeon, and rat aortic smooth muscle. The aorta isolated from the studied animals was cut into 2-3 mm rings, mounted in an organ bath containing physiologic solution and provided with force transducers for measuring the developed isometric tension. The data was recorded using PowerLab Data Acquisition System.
