2023-11

Cytotechnology (القضية : 2023) (الحجم : 2023)

Angiotensin II (Ang II), a mitogen-activated peptide, exerts numerous effects on the cardiovascular system including the regulation of blood pressure. The current study focused on the potential mechanisms that seem to be involved in Ang II vasodilation using bovine aortic endothelial cells (BAE-1) cell lines. Expression of the Ang II receptor (AT2) in BAE-1 was checked by western blots in the presence of valsartan (AT1 inhibitor). To check if Ang II’s vasodilator impact was mediated by the nitric oxide (NO) pathway, the Griess reagent was used. Furthermore, cell-attached patch-clamp and fire-polished borosilicate electrodes with a resistance of 3–5 MΩ in the working solutions was used to record membrane currents from treated BAE-1. BEA-1 revealed 50 kDa immunoreactive bands that matched AT2. The concentration of AT2 was elevated in valsartan-treated cells in comparison to control cells. The biochemical experimental data indicated that the NO level increased in a concentration-dependent manner. Meanwhile, Ang II at a concentration of 1 µM, the level of NO increased more than at 100 µM. In patch-clamp experiments, K current and chord conductance were enhanced after incubation of Ang II with valsartan. When 100 µM Ang II was added, the current peaked rapidly and after 15 min of incubation, the maximum value was obtained, as opposed to 10 min and control (110.9 ± 13.3 pA control, 141.4 ± 30.4 pA after 10 min and 174.4 ± 49.3 pA after 15 min). Ang II type two receptor inhibitor (PD1231777) reduced the current and conductance induced by Ang II. The presented data revealed that Ang II released NO via the activation of AT2. K currents were stimulated by Ang II and evoked mainly a current consistent with the activation of K channels.

2023-09

Egyptian Journal of Veterinary Sciences (القضية : 2) (الحجم : 55)

URTICA DIOICA (UD) is used for a number of therapeutic purposes. Has traditionally been used in the control of cardiovascular disorders, especially hypertension. Also, the leaf extract of UD has been reported to improve glucose homeostasis in vivo. This raises the question of the relationship between UD, diabetes, and heart disorders. Therefore, this study aimed to evaluate the effect of watery leaf extract UD on the heart parameters associated with diabetes mellitus (DM) in Wistar rats. Animals were divided into 4 groups (N = 25). The untreated normal control rats only received distilled water (n = 8).), The diabetic control rats group received alloxan 120 mg/Kg body weight (n = 7), the diabetic rat group treated with UD at 250 mg/kg body weight (n = 5), and the diabetic rats that received UD extract at 500 mg/Kg body weight (n = 5) orally twice daily for 5-6 days. Data showed that in the diabetic group heart rate decreased and induced bradyarrhythmia. After treating diabetic rats with 250 mg/kg enhancement in heart rate was recorded. Additionally, increasing the UD concentration to 500mg/kg further increases the heart rate to the normal value. The current study indicates a widening in QRS, which seems to be disturbances of intraventricular conduction that can be seen in right and left bundle branch blocks, heart failure, and myocardial ischemia. Conclusively, these data show that UD appears to have antidiabetic and noncytotoxic properties, it is associated with reducing the effect of diabetics on the heart

2023-01

International Journal of Occupational Safety and Health (القضية : 1) (الحجم : 13)

Introduction: The rapid and recent rise in the pandemic of cardiovascular disease implies that the environment plays a significant role. Numerous biological systems, such as the cardiovascular, blood-forming organs, liver, and kidneys, can be affected by gasoline and smoking. Because filling station employees, repair service workers, gasoline truck drivers, and refinery workers are all at a greater risk of being exposed to gasoline fumes. Even though gasoline and smoking have been investigated for so many years, few studies have looked into the effects of gasoline exposure combined with smoking on a variety of physiological mechanisms. As a result, we propose that combining gasoline exposure with smoking is a risk factor for cardiovascular diseases and impaired hepatic function. Methods: The study included 95 male adult volunteers who worked with gasoline and were exposed to different fuel derivatives as study group and age and sex-matched seemingly healthy non-exposed people as the controls. Questionnaire interviews were used to collect socio-demographic data and a standard technique was used to collect the blood samples. The levels of cholesterol, HDL4, LDL-C, triglyceride, and VLDL were measured, as well as for liver enzymes ALP, AST, ALT, indirect bilirubin, direct bilirubin, and total bilirubin were measured. Results: Our data suggest that smoking with gasoline exposure causes an increase in total and bad cholesterol levels, as well as a significant shift concerning the control group in lipid profile and liver enzymes. the exposed group had higher levels of ALP, and AST and significantly increased. In the nonsmoker exposed group D-bilirubin decreased in comparison to the control and exposed smoker group. Conclusion: This research concluded that the liver enzymes (ALP, AST, ALT) were higher among workers who smoke and are exposed to gasoline than in control subjects, similarly, the bad cholesterol also increase. Therefore, people who smoke and are handled with gasoline are at a higher risk of having heart and hepatic diseases.

2022-11

Advances in Animal and Veterinary Sciences (القضية : 12) (الحجم : 10)

Various chemical mediators of inflammation such as serotonin, Bradykinin (BK), histamine, substance P, and ATP have been linked to the pathophysiology of multiple vascular ailments, including atherosclerosis, migraine, and myocardial infarction. Cardiovascular performance seems to be significantly regulated by BK. It is also becoming more and more recognized as a component of how medications that affect the liver, kidney, and circulation work. Tetraethylammonium (TEA), a nonselective potassium channel blocker, works as an antiarrhythmic drug in addition to inhibiting potassium channels to affect repolarization. We studied levels of heart electrical activity as well as the physiological effects of different dosages of BK and TEA administered intravenously on a cardiac arrhythmia caused by BaCl2 in vivo. After measuring the electrocardiographic (ECG) parameters in male Wistar rats, which were anesthetized and had electrodes implanted subcutaneously into the right forelimb and left hind limb, the ECG waves and parameters were recorded and analyzed using the LabChart software and the Bio Amp of ADInstruments data acquisition system. Barium Chloride, in doses of 10 and 20 mg/kg (i.v), produced intense and persistent bradycardia associated with prolonged QTc at was observed in the ECG in vivo recording, if not treated after 20-25 in rats were dying. Bradykinin and TEA also produced a similar pattern of a fall in heart rate but inhibited mortality. In addition, the responses produced by TEA are of greater magnitude as compared to the BK. In conclusion, Bk and TEA produce cardioprotective activity in rats.

2022-06

Advances in Animal and Veterinary Sciences (القضية : 6) (الحجم : 10)

Taurine, a ubiquitous endogenous sulfur-containing amino acid, possesses numerous pharmacological and physiological actions, including antioxidant activity, modulation of calcium homeostasis, and antiapoptotic effects. There is mounting evidence supporting the utility of taurine as a pharmacological agent against heart disease, including chronic heart failure. This study investigated the potential protective effects of taurine, on behavior and cardiovascular function in the male rat following arrhythmia induced by the social stressor. Here, we studied levels of heart electrical activity following experimental conditions: Cage control, social isolation in standard rat housing for 14 days, and pharmacokinetics effect of intravenous administration of different doses of taurine on arrhythmia induced by social isolation in vivo after. The ECG signals and parameters were recorded and analyzed with the aid of Bio Amp of ADInstruments data acquisition system and LabChart software. The results showed infusion of Taurine 1mg/kg/hr, 2.5mg/kg/hr and 10mg/kg/hr non significantly change heart rate (BPM), QRS intervals, S amplitude (mV), T amplitude (mV), ST height (mV), JT height (mV), QT intervals (s) and QTc (s).

2019-09

Accepted in the international conference on Toxicology and pharmacology held in Barcelona, Spain (القضية : 6) (الحجم : 7)

Endothelium in blood vessels has an interesting effect on the regulation of blood flow via releasing agents in vascular tissue to manage smooth muscle tone. Endothelium influences blood pressure and has principle effects on the control of local blood flow and vascular permeability by the release of multivariate vascular active factors involving endothelial-derived relaxing and contracting factors to regulate vascular tone. Angiotensin II (Ang II) type 2 receptor (AT2 -R) activation may cause vascular relaxation and thereby it could participate to the antihypertensive responses of Ang II type 1 receptor (AT1 -R) inhibitors. The current study examined whether or not Nitric Oxide (NO) and endothelium mediates relaxation in rat aorta after incubation with the Ang II AT1 -R antagonist valsartan. The data of the current research indicate that Ang II in the presence of valsartan induces vasorelaxation and this response was significantly dependent on protein kinase A (PKA)/endothelium nitric oxide synthase (eNOS) and cyclic guanosine monophosphate (cGMP) after activation of AT2 -R. Furthermore, this study also indicates the interesting role of endothelium in the vasorelaxation induced by Ang II.

2013-07

Journal University of Zakho (القضية : 1) (الحجم : 2)

Sodium Nitroprusside (SNP) and Adenosine (Ado) are potent drugs used in the treatment of cardiovascular diseases. Nitric oxid (NO) is produced from virtually all cell types composing the cardiovascular and regulates vascular function through fine regulation of excitation–contraction coupling. Adinosine endogenous metabolites play a major role in coronary autoregulation. Therefore, the aim of the present study was to investigate the contribution of NO and Ado mediated relaxation in rat aortic smooth muscle in intact and denuded endothelium rings precontracted with phenylepherine (PE). The thoracic aorta was isolated, cut into rings, and mounted in organ-bath chambers and isometric tension was recorded using powerLab Data Acquisition System (Model ML 870). According to the results of the current study, incubation of aortic rings with Glybenclamide (GLIB) decreased the relaxation response induced by Ado (the vasodilation value rate decrease from 41.07±6.7 control to 18.54±4.6) in intact aortic rings. L-nitroarginine methylester (L-NAME), not abolished the response induced by SNP, whereas Nifedipine significantly enhanced the response induced by SNP in a dose-dependent manner in intact endothelium rings. The relaxation to Ado in intact aortic rings was slightly decreased (6.88±1.01), but not abolished completely after incubation with Caffeine (Ado receptors antagonist). On the other hand, removing endothelium did not attenuated the vasorelaxation induced by SNP and increased relaxation response. While, vasorelaxation of Ado in aortic rings were partially attenuated by removing endothelium. These results suggested that (1) ATP-dependent potassium channel (KATP) did not involve in SNP inducing vasorelaxation, while have a role in Ado mediated vasorelation. (2) Vasorelaxation effect of NO is endothelium independent, while, Ado relaxation effect is endothelium dependent.

2013-07

Journal University of Zakho (القضية : 1) (الحجم : 2)

Sodium Nitroprusside (SNP) and Adenosine (Ado) are potent drugs used in the treatment of cardiovascular diseases. Nitric oxid (NO) is produced from virtually all cell types composing the cardiovascular and regulates vascular function through fine regulation of excitation–contraction coupling. Adinosine endogenous metabolites play a major role in coronary autoregulation. Therefore, the aim of the present study was to investigate the contribution of NO and Ado mediated relaxation in rat aortic smooth muscle in intact and denuded endothelium rings precontracted with phenylepherine (PE). The thoracic aorta was isolated, cut into rings, and mounted in organ-bath chambers and isometric tension was recorded using powerLab Data Acquisition System (Model ML 870). According to the results of the current study, incubation of aortic rings with Glybenclamide (GLIB) decreased the relaxation response induced by Ado (the vasodilation value rate decrease from 41.07±6.7 control to 18.54±4.6) in intact aortic rings. Lnitroarginine methylester (L-NAME), not abolished the response induced by SNP, whereas Nifedipine significantly enhanced the response induced by SNP in a dose-dependent manner in intact endothelium rings. The relaxation to Ado in intact aortic rings was slightly decreased (6.88± 1.01), but not abolished completely after incubation with Caffeine (Ado receptors antagonist). On the other hand, removing endothelium did not attenuated the vasorelaxation induced by SNP and increased relaxation response. While, vasorelaxation of Ado in aortic rings were partially attenuated by removing endothelium. These results suggested that (1) ATP-dependent potassium channel (KATP) did not involve in SNP inducing vasorelaxation, while have a role in Ado mediated vasorelation. (2) Vasorelaxation effect of NO is endothelium independent, while, Ado relaxation effect is endothelium dependent.