The Effect of Ethanolic Extract of Stinging Nettle (Urtica Dioica) on the Larval Stages of Greater Wax Moth (Galleria mellonella) Lepidoptera
2023-05
New Valley Journal of Agricultural Science, Egypt (القضية : 2805) (الحجم : 3)
The present study was carried out at the Duhok University College Laboratory Centre in
the Agricultural Engineering Sciences from September 2021 to June 2022, to evaluate the toxic
effect of stinging nettle ethanolic extracts of stinging nettle (Urtica dioica) at different
concentrations on the larval stages of the Greater wax moth (Galleria mellonella, Lepidoptera:
Pyralidae). The Soxhlet extraction method was used in this study, with five concentrations (0.5,
1, 1.5, 2.5, and 3.5 mg/ml) of the ethanolic plant extract after 24, 48, and 72 hours of exposure.
The third and fifth instar larvae of the insect were examined under the conditions (at 30±1°C and
70±5% R.H). There was a highly significant influence (P < 0.001) on concentrations, affected
mortality statistically significant (P < 0.05). The results of the stinging nettle extracted at 3.5
mg/ml after 72 hours of exposure were obtained and the highest mortality rate recorded in the 3rd
and 5th instar larvae which were 96.66 and 86.66% respectively.
The ethanolic Nettle Stinging extract was testified to cause morphological deformations in both
larval instars of the insect
2022
THE EFFECT OF URTICA DIOICA EXTRACTS ON THIRD LARVAL INSTARS OF TRIBOLIUM CONFUSUM (DU VAL) USING TWO METHODS OF EXTRACTION
2022-12
Science Journal of university of Zakho (القضية : 2663) (الحجم : 10)
The effect of ethanolic extracts of Urtica dioica on mortality of the third Larval instar of Tribolium confusum, was studied using
the Soxhlet and solvent extraction methods. Experiments at 30 ± 1°C and 70 ± 5% relative humidity. In laboratory conditions. The
mortality data were recorded after 24, 48, and 72 hours. The mortality rate increased by an increase in concentrations (0.5, 1, 1.5,
and 2.5 mg/ml), the highest mortality rate was 100% at 1.5 mg/ml, while the lowest percentage of mortality 73.33% was observed
after 24 hrs of exposure at 0.5 mg/ml dose by Soxhlet extraction method, whereas the highest mortality rates 100% at 2.5 mg/ml
concentration, while the lowest percentage of mortality 90% was observed after 24 hrs of exposure at 0.5 mg/ml dose by Solvent
extraction methods. The results show that the plant extracts tested at different concentrations were toxic to the larvae of T. confusum,
and both methods of extraction had nearly similar effects on larvae