Toxoplasmosis, an infectious disease caused by the obligate intracellular protozoan parasite, poses varying degrees of risk, ranging from asymptomatic cases in immunocompetent individuals to severe, life- threatening conditions in immunocompromised individuals and developing fetuses, especially when infection occurs during early pregnancy. While the disease is endemic in Iraq, there is a notable lack of precise information regarding its seroprevalence among females of childbearing age and pregnant women, along with associated risk factors in the Zakho district. This cross-sectional study aimed to address this gap by determining the prevalence of anti-Toxoplasma gondii IgG and IgM antibodies using the ELISA assay. The study involved 610 females aged 18-79 years from various residential areas within Zakho district, Iraq. The findings revealed a seroprevalence of 32.46% for anti-Toxoplasma IgG antibodies and 8.86% for IgM antibodies. Significant variations in IgG antibody seroprevalence were observed across different age groups (P=0.008), with the highest prevalence noted among those aged 46-55 years (47.73%). Conversely, IgM antibody seroprevalence, while non-significant (P>0.05), displayed the highest rate of 10.05% among ages 18-25 years. The study identified residence as a variable significantly associated with toxoplasmosis. Additionally, contact with cats, marital status, a history of abortion, and the consumption of homemade food showed significant associations with anti-Toxoplasma IgM antibodies only. These findings strongly suggest that Toxoplasma gondii is a prevalent causative agent of infection in Zakho city, Iraq. This study contributes valuable insights into the seroprevalence and associated risk factors, providing a foundation for targeted interventions and further research in this region.

oxoplasmosisis a neglected foodborne disease, standsas the fourth most frequent cause of hospitalization and the second leading factor behind deaths among immunocompromised individuals and pregnant women who contract the disease at early pregnancy stage. The prevalence of toxoplasma infections among males in Zakho city has remained poorly examined. The current study's primary objective isto estimatethe seroprevalence of Toxoplasma gondii(T. gondii) in males residing in Zakho city, Iraq. Data for analysis were gathered through serological tests and participant questionnaires. Among the 213 participants, 65 (30.52%) and 4 (1.88%) exhibited IgG and IgM anti-Toxoplasma gondiiantibodies, respectively. The currentinvestigation highlighted the prevalence ofT. gondiiinfection within the general population of Zakho city, Iraq. However, the rate of seropositivity of anti-toxoplasma IgG, increased with age, but this increase was non-significant (P>0.05). Also, higher but non-significant seroprevalence rates of toxoplasmosis IgG and IgM Abs were observed with other studied factorssuch as residence, contact with cats, occupation, marital status, and eating at restaurants.Indicatingtothe presence of poor relationships between toxoplasmosis and these demographic factors in males.