2023-05

New Valley Journal of Agricultural Science, Egypt (Issue : 2805) (Volume : 3)

The present study was carried out at the Duhok University College Laboratory Centre in the Agricultural Engineering Sciences from September 2021 to June 2022, to evaluate the toxic effect of stinging nettle ethanolic extracts of stinging nettle (Urtica dioica) at different concentrations on the larval stages of the Greater wax moth (Galleria mellonella, Lepidoptera: Pyralidae). The Soxhlet extraction method was used in this study, with five concentrations (0.5, 1, 1.5, 2.5, and 3.5 mg/ml) of the ethanolic plant extract after 24, 48, and 72 hours of exposure. The third and fifth instar larvae of the insect were examined under the conditions (at 30±1°C and 70±5% R.H). There was a highly significant influence (P < 0.001) on concentrations, affected mortality statistically significant (P < 0.05). The results of the stinging nettle extracted at 3.5 mg/ml after 72 hours of exposure were obtained and the highest mortality rate recorded in the 3rd and 5th instar larvae which were 96.66 and 86.66% respectively. The ethanolic Nettle Stinging extract was testified to cause morphological deformations in both larval instars of the insect

2022-12

Science Journal of university of Zakho (Issue : 2663) (Volume : 10)

The effect of ethanolic extracts of Urtica dioica on mortality of the third Larval instar of Tribolium confusum, was studied using the Soxhlet and solvent extraction methods. Experiments at 30 ± 1°C and 70 ± 5% relative humidity. In laboratory conditions. The mortality data were recorded after 24, 48, and 72 hours. The mortality rate increased by an increase in concentrations (0.5, 1, 1.5, and 2.5 mg/ml), the highest mortality rate was 100% at 1.5 mg/ml, while the lowest percentage of mortality 73.33% was observed after 24 hrs of exposure at 0.5 mg/ml dose by Soxhlet extraction method, whereas the highest mortality rates 100% at 2.5 mg/ml concentration, while the lowest percentage of mortality 90% was observed after 24 hrs of exposure at 0.5 mg/ml dose by Solvent extraction methods. The results show that the plant extracts tested at different concentrations were toxic to the larvae of T. confusum, and both methods of extraction had nearly similar effects on larvae