Education
Professional Experience
* Research interest: Molecular Medicine, Molecular Biology, Medical Physiology, Oncology, Biotechnology, Cell Biology, Biochemistry, and Genetics.
* Medical and Molecular techniques and methods: o PCR: qPCR, RT-PCR, and conventional PCR, o Immunoblotting: Western blotting, ELISA o Sequencing methods: Chip-Seq, RIP-seq, and RNA sequencing o Immunostaining: Immunocytochemistry o Cell and Tissue culture: cell culture of adherent and suspension cells, Cryopreservation. o Assays: Wound healing, migration, invasion, and proliferation assay. o Primer and Probe designing o Extraction methods: Nucleic acid extraction and protein extraction o Transfection (lipofectamine) and transformation. o Gene knockdown (RNA silencing) o Electrophoresis: PAGE, Agarose Gel Electrophoresis. o Microscope: Confocal Microscope (nondependent), widefield fluorescence microscope, Light Microscope, Dark-field Microscopy, Dissecting Microscope. o Others: Spectrophotometer, Nanodrop, Plate reader, Sonication, Autoclaving ...etc.
* Medical and Molecular Computer Program: o GraphPad prism: for Statistical analysis o PhotoScape and ImageJ: Image analyzer for multiple techniques o Image Lab: Western blot analysis o Snap gene and Serial Cloner: for gene manipulation o Quant Studio and Step One: For qPCR o ZEN: for confocal microscopy o Nanodrop Program: Nucleic acid quality and quantity detection. o Plate reader program o IBS and Biorender: Create figure, sketch, and diagrams. o Mendeley and RefWorks: referencing programs. o Excel: for Analyzing NGS data, qPCR fold enrichment, RIP-seq, Chip-seq fold enrichment.