English العربية کوردی

ئەز   Nidhal Meena Sher Mohammed

Assistant prof.

Specialties

PhD instrumental analysis/ Analytical Chemistry

nidhal.mohammed@uoz.edu.krd
07500640768
Iraq , Zakho
1
Awards
18
Published Journal Articles
1
Thesis
0
Training Course

0
Book Chapter
10
Conference
4
Presentation
6
Workshop

Education

PhD instrumental analysis

Glasgow/UK لە Glasgow/UK

2012

MSc analytical chemistry

Chemistry لە Bagdad

2003

BSc Chemistry

Chemistry لە Baghdad

1991

Membership


2009

2009-03-04,2013-03-04
membership

Association of Applied Biologist (AAB), UK.

2009-02-01,2013-02-04
membership

The Royal Society of Chemistry (RSC), London, UK.

2008

2008-02-11,2013-02-11
memership

Society of Chemical Industry (SCI), London, UK.

Academic Title

Assistant prof.

2017-06-15

Lecturer

2012-10-22

Assis. Lecturer

2003-12-03

Awards

لجنة لقب علمي

2021-05
1312

عمل وجهود اعضاء اللجنة

 2021

Published Journal Articles

International Journal of Environmental Science and Technology
Preparation and characterization of potato peel derived hydrochar and its application for removal of Congo red: a comparative study with potato peel powder

This study aimed to investigate the potential of potato peel waste powder as a biomass... See more

This study aimed to investigate the potential of potato peel waste powder as a biomass feedstock for hydrothermal carbonization to produce hydrochar, which could be used as an adsorbent for water treatment. An autoclave was used at 200 °C for 25 h to prepare potato peel hydrochar from potato peel waste powder and compare their physicochemical properties using various characterization techniques. The results showed that potato peel hydrochar had a higher specific surface area and adsorption capacity for Congo red than potato peel waste powder, making it a viable adsorbent for water treatment. The experimental data were well-fitted with the Freundlich isotherm and pseudo-second-order kinetic models, suggesting that the adsorption process at the solid/solution interface is spontaneous, endothermic, and more disordered. The study highlights the potential of waste biomass, such as potato peel, to produce hydrochar as an effective and sustainable adsorbent for water treatment.

 2023-05
New Journal of Chemistry (Issue : 16) (Volume : 47)
Synthesis of MnO2 sub-microspheres with effective oxidase-mimicking nanozymes for the colorimetric assay of ascorbic acid in orange fruits and juice

Nanozymes, as an alternative to natural enzymes, have the advantages of good catalytic efficiency, high... See more

Nanozymes, as an alternative to natural enzymes, have the advantages of good catalytic efficiency, high stability, easy storage, low-cost, and the ability to be used under extreme conditions compared with natural enzymes. Improving the sensitivity of nanozyme-based assays is highly required and depends on the nanozyme size, chemical structure and morphology. In the present work, an oxidase-mimicking nanozyme of MnO2 sub-microspheres was synthesized from KMnO4 and MnCl2·4H2O via a one-step hydrothermal method followed by calcination for 2 h at 300 °C. The oxidase-like activity of the sub-microspheres was evaluated through the conversion of 3,3′,5,5′-tetramethylbenzidine (TMB; colorless) to (ox-TMB (blue color). The kinetic results showed that the catalytic behavior of the MnO2 sub-microspheres follows Michaelis–Menten kinetics with a low constant (Km = 0.1 mM) compared with the natural enzyme HRP (Km = 0.434 mM). The catalytic mechanism was deduced, whereby the oxidase-mimicking behavior is dependent on the formation of O2˙− radicals and partially dependent on OH˙ radicals. An efficient colorimetric platform capable of sensitive determination of ascorbic acid was developed based on fading of the blue color of ox-TMB by ascorbic acid. The linear detection range was from 1.0 to 100 μM, and the limit of detection was 0.33 μM. The proposed method showed high selectivity and sensitivity for the detection of ascorbic acid. Further applications in real samples were also successfully demonstrated.

 2023-03
AIP publishing (Issue : 1) (Volume : 2660)
Assessment physic-chemical quality of drinking water of Bardarash district/Duhok city in Kurdistan Region of Iraq

Safe quality of drinking water is a critical concern for human health. Bardarash is one... See more

Safe quality of drinking water is a critical concern for human health. Bardarash is one of the districts of Duhok city located in the Kurdistan Region in the north part of Iraq and considered a rich area of oil resources. Groundwater wells are the main sources of drinking water in this area. To date, no study has focused on examining the quality of drinking water in Bardarash district. The aim of this study is to assess the physic-chemical quality of drinking water in this area. Water samples were collected from local wells (103) from four subjoined districts and analyzed for 10 heavy metals and physico-chemical parameters using atomic spectroscopy and standard methods. The results showed that most of the heavy metals and physico- chemical analysis values were below the permissible limits set by WHO, while few values were higher for Al, Fe, Cr, NO31- and TAL. The data also show significant variation (P<0.05) of the physico-chemical parameters and some heavy metals among four subjoined districts. It can be concluded that the water samples were not highly contaminated although water treatment with regular monitoring and periodic cleaning of domestic tanks is required to obtain sustainable clean drinking water in this area.

 2022-11
International Journal of Environmental Analytical Chemistry (Issue : 15) (Volume : 102)
Green synthesis and characterisation of monometallic (Ni) and bimetallic (Ni-Ag) nanoparticles using Cicer Arietinum leaf extract and their applications for adsorption of toluidine blue

In this study, monometallic (Ni) and bimetallic (Ni-Ag) nanoparticles were synthesised by a bio reduction... See more

In this study, monometallic (Ni) and bimetallic (Ni-Ag) nanoparticles were synthesised by a bio reduction approach using Cicer Arietinum leaf extract which is known to contain high amount of phenolic and flavonoid compounds that can serve as nano-bio sources for synthesis metallic NPs. Biosynthesised nanoparticles were characterised using various techniques viz UV-Vis, FE-SEM, EDX, and XRD. The average crystallite diameter was found to be 30 nm and 24 nm for NiNPs and Ni-AgNPs, respectively, with almost spherical shapes, regular morphology and homogenously formed. The synthesised NPs were successfully applied for adsorption of toluidine blue dye (TBO) in aqueous solution using batch experiments with different process parameters namely initial dye concentration, contact time, pH, adsorbent dosage, and temperature. The adsorption isotherms and kinetics were investigated in detailed; the results followed Freundlich model (R2 = 0.990, 0.976) and pseudosecond- order kinetic (R2 = 0.998, 0.999) for both mono and bimetallic NPs, respectively. Finally, adsorption process showed that bimetallic Ag-NiNPs (qe = 94 mg g−1) could be considered mor

 2022-09
Analytical Biochemistry (Issue : 114818) (Volume : 654)
Enhancing enzymatic activity of Mn@Co3O4 nanosheets as mimetic nanozyme for colorimetric assay of ascorbic acid

In nanozyme-based assays, increasing enzymatic activity is very desirable for enhancing sensitivity and lowering the... See more

In nanozyme-based assays, increasing enzymatic activity is very desirable for enhancing sensitivity and lowering the detection limit. In this study, novel Mn doped cobalt oxide nanosheets (Mn@Co3O4 NSs) were synthesized by hydrothermal process. The obtained Mn@Co3O4 possessed enhanced dual-enzyme mimetic, oxidase and peroxidase, and can catalytically oxidize of 3, 3′ , 5, 5′ -tetramethylbenzidine (TMB), to a blue product of oxidized TMB. The enzyme kinetics were well-described mathematically using a common Michaelis-Menten and Lineweaver Burk model. The enzyme kinetics constant (Km) was found to be 0.15 mM, which is relatively low comparing with pure Co3O4 nanosheets (0.35 mM) and natural enzyme HRP (0.434 mM). Therefore, the efficient colorimetric method was achieved for determination of H2O2 and ascorbic acid. The limit of detection (LOD) of H2O2 was 8.0 μM and the linear range was 20–200 μM based on direct turn on of the peroxidase-like activity of Mn@Co3O4. While, for ascorbic acid detection based on turn-off approach, the linearity range for the ascorbic acid was 1–8 μM with LOD of 0.4 μM. Moreover, the colorimetric system exhibited good stability and selectivity toward the detection of ascorbic acid effectively in real samples (vitamin C tablets) with satisfactorily accuracy and precision.

 2022-07
Science journal of university of Zakho (Issue : 1) (Volume : 9)
A Nanodrop Spectrophotometric Method and Stability Indicating for Determination of Amlodipine Besylate in Pharmaceutical Formulations of Kurdistan of Iraq

ABSTRACT: A nanodrop spectrophotometric method was developed and validated for determination of amlodipine besylate (AB)... See more

ABSTRACT: A nanodrop spectrophotometric method was developed and validated for determination of amlodipine besylate (AB) in bulk and tablet dosage form. The maximum absorption of amlodipine was shown at 357 nm using acetonitrile as a solvent. The developed method was found to be linear (R2 = 0.9990) within the concentration range of 2-10 μg/mL. The precision study showed acceptable values of RSD% (less than 1%). LOD and LOQ values were found to be 0.34 and 1.14 μg/mL, respectively. Accuracy study showed good recovery 99% Awalodipine and 98.88% Amloneer, in locally commercial tablets. The present method was applied successfully for stability indicating study of AB inA walodipine and Amloneer products manufacturedi n (Erbil and sulaymaniyah, respectively)/ Kurdistan of Iraq. The stability -indicating study was investigated under acidic, basic, o xidative, photolytic, and thermal conditions. The results of both products showed that AB is unstable in acidic, alkaline, and oxidative conditions under heating at 60℃ up to 5 hr.s While under photolytic and thermal conditions, the degradation percentagwe as less than 15% indicating to the stability of AB in both Awalodipine and Amloneer tablets according to International Conference on Harmonization (ICH) guideline of drugs. It can be concluded that the main factor that affects the degradation of AB is the passage of time.

 2021-03
Asian Journal of Applied Chemistry Research (Issue : 2) (Volume : 6)
Development Method for Spectrophotometric Analysis of Sulfamethoxazole Using Vanilline Reagent

This study involves a development method for spectrophotometric analysis of sulfamethoxazole (SMZ) with vanillin reagent... See more

This study involves a development method for spectrophotometric analysis of sulfamethoxazole (SMZ) with vanillin reagent in acidic medium. The reaction mechanism was proposed by Schiff's base reaction of the amine group in SMZ with the carbonyl group in vanillin. The spectrum of SMZ complex showed high absorbance at 399.09 nm following the Beer–Lambert law in the concentration levels ranging from 5 to 80 μg.mL-1 with good sensitivity. The stability constant of the product was determined. The values of relative standard deviation (≤ 6.63) and recovery (> 98.73%) indicated a reasonably precise and accurate method with a good limit of detection and quantification. The present method was compared with other spectrophotometric methods in the literature. The procedure of the method showed to be simple since no need for temperature control and solvent extraction. The suggested method was used to determine of SMZ in commercial pharmaceutical drops and compared with the certified method

 2020-07
International Journal of Pharmaceutical Sciences and Research (Issue : 1) (Volume : 10)
METHOD DEVELOPMENT AND VALIDATION OF SIMULTANEOUS DETERMINATION OF HYDROCHLOROTHIAZIDE AND LOSARTAN IN TABLET DOSAGE FORM BY RP-HPLC

Fixed-dose combinations have several advantages comparing with a single drug and separate agents. For simple... See more

Fixed-dose combinations have several advantages comparing with a single drug and separate agents. For simple dosing regimens and their synergistic antihypertensive action, a combination of hydrochlorothiazide and losartan potassium is widely prescribed. In this study, an HPLC-UV method was developed and validated for the simultaneous determination of hydrochlorothiazide and losartan in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of 60: 40 acetonitrile: water, ACE3-C18 column (250 mm × 4.6 mm 5 µm), 20 µl injection volume, the flow rate of 1 ml/min at ambient temperature (25 °C), and 226 nm. The method was validated giving good precision (RSD% < 1), acceptable linearity (R2 ≥ 0.997), and low LOD and LOQ (0.5 and 1.5 µg/ml, respectively). Successful application on pharmaceutical dosage tablet form gave recovery percent within acceptance criteria (92% and above) indicating that the proposed method is simple and reliable for the determination of LOP and HCTZ and hence can be applied for routine analysis in quality control laboratories.

 2019-06
sjuoz (Issue : 2) (Volume : 7)
OPTIMIZATION OF ARSENIC ADSORPTION ONTO ACTIVATED CARBON OF POTATO PEEL USING RESPONSE SURFACE METHODOLOGY

The present study was designed to optimize the adsorption of arsenic onto potato peel derived... See more

The present study was designed to optimize the adsorption of arsenic onto potato peel derived activated carbon (MPP-AC) by employing response surface method and central composite design. Adsorbent of cheap and locally available potato residue was produced based on chemical activation with H3PO4 subsequently carbonization to produce the porous activated carbon. The individual and interactive effects of five variables including initial arsenic concentration, temperature, time, dosage amount and pH of the medium were investigated. Based on the statistical analysis (ANOVA), the quadratic model was developed associating the adsorption capacity (qe). The optimum conditions were obtained of 9.98 mg L-1 of initial As (V) concentration, 28 °C of temperature, 39.7 min of time, 0.97 g of adsorbent dose and 7.3 of pH. The maximum adsorption capacity was 0.27 mg g-1 and 76.5% removal efficiency. The equilibrium isotherms and kinetic studies for estimating the mechanism of process demonstrated a good fit to Langmuir model and the pseudo-second order, respectively. The results of this study showed that the feasibility of central composite design (CCD) to control adsorption process and indicated the use of activated carbon of potato residue have important implications for As (V) removal.

 2019-06
International Journal of Pharmaceutical Sciences and Research, (Issue : 1) (Volume : 10)
Development And Validation Of Simultaneous Determination Of Hydrochlorothiazide And Losartan In Tablet Dosage Form BY RP-HPLC

Fixed-dose combinations have several advantages comparing with a single drug and separate agents. For simple... See more

Fixed-dose combinations have several advantages comparing with a single drug and separate agents. For simple dosing regimens and their synergistic antihypertensive action, a combination of hydrochlorothiazide and losartan potassium is widely prescribed. In this study, an HPLC-UV method was developed and validated for the simultaneous determination of hydrochlorothiazide and losartan in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of 60: 40 acetonitrile: water, ACE3-C18 column (250 mm × 4.6 mm 5 μm), 20 μl injection volume, the flow rate of 1 ml/min at ambient temperature (25 °C), and 226 nm. The method was validated giving good precision (RSD% < 1), acceptable linearity (R2 ≥ 0.997), and low LOD and LOQ (0.5 and 1.5 μg/ml, respectively). Successful application on pharmaceutical dosage tablet form gave recovery percent within acceptance criteria (92% and above) indicating that the proposed method is simple and reliable for the determination of LOP and HCTZ and hence can be applied for routine analysis in quality control laboratories.

 2019-01
Science journal of university of Zakho (Issue : 3) (Volume : 5)
Adsorption of Cr(VI) from Aqueous Solutions using Powder of Potato Peels as a Solid Waste

Pollution of wastewater with heavy metal has always been a serious problem to the environment.... See more

Pollution of wastewater with heavy metal has always been a serious problem to the environment. Chromium is considered one of the most noxious heavy metals. Adsorption is now reorganized as an alternative technology of defence for chromium removal due to local availability, technical efficiency and cost effectiveness. Potato peel powder can be used as a low cost biosorbent to remove hexavalent chromium from aqueous solutions under various experimental conditions. Different parameters including equilibrium contact time, initial metal ion concentration, potato peel dose, pH and temperature were studied through a number of batch sorption experiments. Both the Langmuir and Freundlich were found to fit the adsorption isotherm of Cr (VI) ion onto potato peel. The Langmuir adsorption capacity was found to be 1.97 mg/g while Freundlich constants including Kf and n were 1.57 and 2.5, respectively. The adsorption kinetic was found to be more fit with the pseudo-first order model. This study showed a high efficiency of potato peel for the biosorption of Cr (VI) ion from aqueous solutions.

 2017-09
International Journal of Pharmaceutical Sciences and Research (Issue : 6) (Volume : 8)
Method Development And Validation Of Atenolol Using Two HPLC Systems

A reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the... See more

A reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for the determination of Atenolol (ATN) in bulk and pharmaceutical formulation. The method was optimized selecting chromatographic conditions of 60: 40 acetonitrile: water, Inertsil_ column(ODS-3 250 mm × 4.6 mm 5 𝜇m), 20 𝜇L injection volume, flow rate of 1 mL/min at ambient temperature (30 °C), and 276 nm. Using two HPLC systems of first HPLC system coupled with PDA detector and the second HPLC system coupled with UV detector showed no big difference in the method results. The method was validated giving good precision (RSD% < 1), acceptable linearity (R2 > 0.998), and low LOD and LOQ (0.5 and 1.5 𝜇g/mL, respectively) on both systems. Successful application on pharmaceutical dosage tablet form gave high recovery of 97.7%. The proposed method is economic, simple, and rapid and hence can be employed for routine analysis in quality control laboratories.

 2017-06
Chromatography Research International (Issue : 2016) (Volume : 2016)
Development and Validation of RP-HPLC Method for the Determination of Hydrochlorothiazide in Bulk Drug and Pharmaceutical Dosage Form

An HPLC-PDA method was developed and validated for the determination of hydrochlorothiazide in bulk and... See more

An HPLC-PDA method was developed and validated for the determination of hydrochlorothiazide in bulk and pharmaceutical formulation.Themethod was optimized selecting chromatographic conditions of 50 : 50 acetonitrile :water, Inertsil column(ODS- 3 250mm × 4.6mm 5 𝜇m), 20 𝜇L injection volume, flow rate of 1 mL/min at ambient temperature (30∘C), and 272 nm. Another column of C18Zorbax (Eclipse Plus, 4.6 × 250 mm, 5 𝜇m) was tested showing no big difference in the method results.The method was validated giving good precision (RSD% < 1), acceptable linearity (𝑅 2 ≥ 0.9978), and low LOD and LOQ (0.5 and 1.7 𝜇g/mL, resp.) on both columns. Successful application on pharmaceutical dosage tablet form gave high recovery of 99.93%. The method was compared with official BP and other reported methods.The proposed method is economic, simple, and rapid and hence can be employed for routine analysis in quality control laboratories.

 2016-08
Indian Journal of Materials Science (Issue : 2016) (Volume : 2016)
Adsorption of 3-Chloroaniline on Potato Skin in Aqueous Solution

Theadsorption behaviour of aromatic amine 3-chloroaniline (3-CA) fromaqueous solution on fresh potato skin was investigated.... See more

Theadsorption behaviour of aromatic amine 3-chloroaniline (3-CA) fromaqueous solution on fresh potato skin was investigated. A series of batch experimentswere conducted under different experimental conditions of contact time, 3-chloroaniline concentration, weight of potato skin, pH, temperature, and ionic strength using RP-HPLC analysis. Adsorption equilibrium of 3-chloroaniline at concentration of 10 𝜇g/mL on 1 g weight of chopped potato skin was achieved in 24 hours. Using different varieties of potato skin showed that the adsorption of 3-CA on Nicola variety is higher compared to Sante and Maris Peer varieties. Adsorption on potato skin was found to be generally higher compared to cortex and pith tissues. Analysis of adsorption isothermshows that equilibrium data was fitted to Freundlich model (𝑅 2 = 0.977). Maximum adsorption capacities of 3-chloroaniline were found in the pH range from3 to 9,whereas lowadsorption quantitieswere found in high acidic and high basic solutions (pH 2 and pH13, resp.).Adsorption capacity increased with an increase in temperature from 4∘C to 30∘C but decreased with further increase of temperature to 40∘C. Testing the ionic strength showed that increasing the concentration of electrolyte reduces the adsorption efficiency. This study indicated that the fresh potato skin (without any treatment) is possible to use as a new adsorbent for removal of 3-chloroaniline from industrial waste water.

 2016-07
Eur. Chem. Bull. (Issue : 8) (Volume : 4)
SPECTROPHOTOMETRIC DETERMINATION OF OXYMETAZOLINE HYDROCHLORIDE VIA CHARGE TRANSFER REACTION USING 2,3-DICHLORO-5,6-DICYANO1,4-BENZOQUINONE (DDQ) REAGENT

Simple, sensitive and reproducible spectrophotometric method was developed for estimation of oxymetazoline hydrochloride via charge... See more

Simple, sensitive and reproducible spectrophotometric method was developed for estimation of oxymetazoline hydrochloride via charge transfer complex formation reaction. The method is based on the reaction of oxymetazoline as n-donor with DDQ reagent as π-acceptor in basic aqueous medium of pH 9.79. The maximum absorption is 421 nm. Beer's law was obeyed in the range 0.4-4.0 µg mL-1 with molar absorptivity 2.5 x 104 L mol-1 cm-1. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.0093 and 0.028 µg mL-1 respectively. The stability constant has been determined and the mechanism of the reactions was proposed. The proposed method was applied to determine the oxymetazoline hydrochloride in dosage form as drop, and the results were statistically compared with official BP method.

 2015-06
IOSR Journal of Environmental Science, Toxicology and Food Technology (Issue : 4) (Volume : 9)
HPLC-UV Method for the Analysis of Potato Sprout Inhibitor Chlorpropham and Its Metabolite 3-Chloroaniline in Potatoes

A simple extraction method based on methanol-soaking coupled with HPLC-UV detection was developed for the... See more

A simple extraction method based on methanol-soaking coupled with HPLC-UV detection was developed for the simultaneous determination of the potato sprout inhibitor chlorpropham (isopropyl 3-chlorophenyl carbamate) (CIPC) and its degradation product 3-chloroaniline (3-CA) in potato samples using propham (IPC) as an internal standard. The chromatographic conditions selected were 62% methanol at 15 minutes run time and retention times at 11 and 4 minutes for CIPC and 3-CA respectively. The HPLC method was validated reporting good precision and acceptable linearity. The estimated values of LOQ in the organic potato extract based on fresh potato tuber weight were 0.01 and 0.02 mg/kg of CIPC and 3-CA respectively. The initial extraction using overnight methanol-soaking gave recovery values of up to 90% for CIPC following spiking organic potato peel for 1 hour at spiking levels of 0.8 and 8.0 μg/g. However, 3-CA recovery was found to be very low (< 10%) particularly at the lower level of 0.8 μg/g when no 3-CA was detected. To improve the extractability of 3-CA, the extracting solution of methanol was acidified by adding sulphuric acid. Optimising the extraction process showed that using 1 M sulphuric acid prepared in 50% methanol as an extracting solution for 24 hours at 50 ºC improved the extraction recovery of 3-CA up to 85% at a spiking level of 8.0 μg/g and contact time 1 hour. This final extraction method was applied for the determination of the residues of both CIPC and 3-CA in commercial potato samples which had received several applications of CIPC reporting high residue values of 3-CA whereas CIPC residues were lower than maximum residue level (MRL) of 10 mg/kg. Keywords: Potato, Chlorpropham; 3-Chloroaniline; Sprout inhibitor; HPLC

 2015-04
Chromatography Research International (Issue : 2014) (Volume : 2014)
Development and Validation of an HPLC Method for the Analysis of Chlorpropham and 3-Chloroaniline in Potato Extracts

Chlorpropham (CIPC) is the main sprout inhibitor used by potato industry.There is concern about the... See more

Chlorpropham (CIPC) is the main sprout inhibitor used by potato industry.There is concern about the residues of CIPC and its degradation product 3-chloroaniline, 3-CA; hence, analytical methods are required to analyse their residues in potato samples. An HPLC-UVmethod was developed and validated for the separation and quantification of these compounds using propham(IPC) as an internal standard.The chromatographic conditions required to achieve good separation were 60%mobile phase of methanol, 15- minute run time at a flow rate of 1.5 mL/min, and a detection wavelength of 210nm using Phenomenex (ODS-2 250mm× 4.60mm 5 𝜇m Sphereclone) column at an ambient temperature. The method was validated for precision, linearity, the limit of detection (LOD) and the limit of quantification (LOQ), producing high precision through RSD ≤ 0.03%, and acceptable criteria of the coefficient of determination (𝑅 2) of the calibration curves (0.990). LOD values of CIPC and 3-CA were approximately 0.01 𝜇g/mL whereas the LOQ values were approximately 0.04 𝜇g/mL using repeated injection approach. The proposed HPLC method was compared with the standard GC method of the CIPC residues extracted showing good agreement (𝑅 2 = 0.99). Despite using the same extract, the recovery results for the proposed HPLC method were 13% higher than GC analysis.

 2014-12
Chromatography Research International (Issue : 2014) (Volume : 2014)
HPLC-UV Method Development and Validation of Potato Sprout Inhibitor 1,4-Dimethylnaphthalene Using Different Systems

1,4-Dimethylnaphthalene (1,4-DMN) is effective sprout suppressant used in potato stores in many countries in the... See more

1,4-Dimethylnaphthalene (1,4-DMN) is effective sprout suppressant used in potato stores in many countries in the world. High residue levels of this compound on the potatoes and in other environmental samples are considered for human health and environmental risks. Determination of the residue requires specific analytical methods to be developed and validated. In this study, HPLC-UV was selected for validating a separation method based on reversed phase for the analysis of 1,4-DMN using 2-methylnaphthalene (2-MeN) as internal standard testing three HPLC systems. Under the same chromatographic conditions, all three systems achieved good separation on a Jones column (Hypersil ODS 5 𝜇m, 250mm× 4.6 mm) at ambient temperature isocratically using 70% acetonitrile as mobile phase at a flow rate of 1.5mLmin−1, 20𝜇L injection volume, a run time of 10 min, and a detection wavelength of 228 nm. All three systems showed high precision, good linearity, and low limit of detection (LOD) and quantification (LOQ); particularly, the SpectraSERIES UV100-autosampler system offered lower values of LOD (0.001– 0.004 𝜇gmL−1) and LOQ (0.002–0.013 𝜇gmL−1) for both compounds. This system can be used for the quantitative determination of 1,4-DMN residue in potato and environmental samples.

 2014-09

Thesis

2012-06-22
Extraction And HPLC Analysis Of Potato Sprout Suppressant Chemicals

Phd Thesis

 2012

Conference

4TH INTERNATIONAL CONFERENCE ON MATERIALS ENGINEERING & SCIENCE (IConMEAS 2021)
 2022-11
Assessment physic-chemical quality of drinking water of Bardarash district/Duhok city in Kurdistan Region of Iraq

Safe quality of drinking water is a critical concern for human health. Bardarash is one of the districts of Duhok city located in the Kurdistan Region in the north part of Iraq and considered a... See more

Safe quality of drinking water is a critical concern for human health. Bardarash is one of the districts of Duhok city located in the Kurdistan Region in the north part of Iraq and considered a rich area of oil resources. Groundwater wells are the main sources of drinking water in this area. To date, no study has focused on examining the quality of drinking water in Bardarash district. The aim of this study is to assess the physic-chemical quality of drinking water in this area. Water samples were collected from local wells (103) from four subjoined districts and analyzed for 10 heavy metals and physico-chemical parameters using atomic spectroscopy and standard methods. The results showed that most of the heavy metals and physico- chemical analysis values were below the permissible limits set by WHO, while few values were higher for Al, Fe, Cr, NO31- and TAL. The data also show significant variation (P<0.05) of the physico-chemical parameters and some heavy metals among four subjoined districts. It can be concluded that the water samples were not highly contaminated although water treatment with regular monitoring and periodic cleaning of domestic tanks is required to obtain sustainable clean drinking water in this area.

International Conference on Advanced Science and Engineering 2018 (ICOASE2018) sponsored by IEEE Iraq Section
 2018-10
Participated as conference committee member

Participated as conference committee member

The 1st International Conference on materials engineering & science (IConMEAS 2018)
 2018-08
Listener

Listener

2nd INTERNATIONAL SCIENTIFIC CONFERENCE – UNIVERSITY OF ZAKHO
 2017-04
Adsorption of Cr(VI) from Aqueous Solutions using Powder of Potato Peels as a Solid Waste

Heavy metal pollution in wastewater has always been a serious environmental problem. Chromium is considered one of the most noxious heavy metals. Adsorption is now reorganized as an alternative technology of defence for chromium removal... See more

Heavy metal pollution in wastewater has always been a serious environmental problem. Chromium is considered one of the most noxious heavy metals. Adsorption is now reorganized as an alternative technology of defence for chromium removal because of cost effectiveness, local availability and technical feasibility. Potato peel powder was used as a low cost biosorbent in Zakho city to remove hexavalent chromium from aqueous solution under various operating conditions. The effect of different parameters such as contact time, initial metal ion concentration, potato peel dose, pH and temperature was investigated through a number of batch sorption experiments. Both the Langmuir and Freundlich were found to fit the adsorption isotherm of Cr (VI) onto potato peel. The Langmuir adsorption capacity was found to be 1.97 mg/g while Freundlich constants Kf and n were 1.57 and 2.5, respectively. The adsorption process can be described by the pseudo-first equation. The results revealed that potato peel is very effective for the biosorption of Cr (VI) from aqueous solutions.

The First International SQU Chemistry Conference
 2015-11
Spectrophotometric Determination of Sulfamethoxazole via Schiffs Base Reaction Using Vanillin Reagent

Participated in a poster presentation

The first conference to honour the graduating students sponsored by ministry of higher education in Iraq, October 2013, University of Baghdad, Iraq.
 2013-10
Participatant

Participatant

Modern Fungicides and Antifungal Compounds VI: Proceedings 16th International Reinhardsbrunn Symposium on Modern Fungicides and Antifungal Compounds
 2011-05
Determination of the potato sprout inhibitor chlorpropham and its metabolite 3-chloroaniline in potatoes samples

A simplified method based on soaking overnight extraction coupled to HPLC - UV analysis was developed for the simultaneous determination of the residue levels of the potato sprout inhibitor chlorpropham (CIPC) and its metabolite 3-chloroaniline... See more

A simplified method based on soaking overnight extraction coupled to HPLC - UV analysis was developed for the simultaneous determination of the residue levels of the potato sprout inhibitor chlorpropham (CIPC) and its metabolite 3-chloroaniline (3CA) in potato samples. The method gave values approximately 25% higher when compared with a standard Soxhlet –GC method. The results of spiking different layers from the potato tuber showed a high recovery of CIPC (> 95%) in all layers but the recovery of its metabolite 3CA was lower than 50% in the pith and 5% in both cortex and skin.

Third Scottish Postgraduate Symposium on Environmental Analytical Chemistry
 2010-12
EXTRACTION OF THE RESIDUES OF CHLOPROPHAM AND ITS METABOLITE 3-CHLOROANILINE IN TREATED POTATOES USING VARIOUS METHODS

In the UK potatoes destined for the processing and fresh pack sectors have to be stored for up to 9 months. To be able to supply potatoes of suitable quality throughout this period it is... See more

In the UK potatoes destined for the processing and fresh pack sectors have to be stored for up to 9 months. To be able to supply potatoes of suitable quality throughout this period it is essential to hold the processing potatoes at temperatures of the order of 10 degrees centigrade. At such temperatures sprouting will occur thereby necessitating the use of a sprout suppressant treatment. The almost universal approach is to employ chlorpropham (CIPC) for this purpose. The application is generally made using a hot fogging technique whereby the fine particles of CIPC produced are distributed throughout the potato store. Several CIPC formulations are available which are aspirated into a stream of hot air at temperatures in excess of 450 oC. Applications may be repeated throughout the storage season. The total amount of chemical that can be applied is restricted to less than 63.75 mg/kg for potatoes destined for processing and 36 mg/kg for fresh pack potatoes. Difficulties are experienced with evenness of application of the chemical and making sure that the MRL value of 10mg/kg is not exceeded. Concerns have been expressed regarding the susceptibility of CIPC to breakdown at high temperatures. This is a particular concern where the application temperature exceeds 450 oC as the literature indicates that thermal breakdown of carbamates such as CIPC is highly likely. The main breakdown product 3-chloroaniline (3-CA) has been detected in store atmospheric samples collected shortly after thermal fogging. Claims have also been made that it is present on the treated potato samples although findings have been inconclusive to date. On investigating this it soon became clear that difficulties existed in developing a suitable analytical method for measuring 3-CA in potatoes The work reported here describes the development of a method for 3-CA determination in potatoes including the simultaneous measurement of CIPC.

Second Scottish Postgraduate Symposium on Environmental Analytical Chemistry
 2009-12
Extraction of 3-Chloroaniline from Potato Tubers

Oral presentation

First Scottish postgraduate symposium on environmental analytical chemistry, University of Glasgow, UK.
 2008-12
Assessment of LOD and LOQ for 1, 4-Dimethylnaphthalene analysis by HPLC

In the UK potatoes for processing into chips, crisps, etc, are stored for up to one year to ensure continuity of supply. To prevent sprouting in store, chemical sprout inhibitors are used. Dimethylnaphthalene (DMN) is... See more

In the UK potatoes for processing into chips, crisps, etc, are stored for up to one year to ensure continuity of supply. To prevent sprouting in store, chemical sprout inhibitors are used. Dimethylnaphthalene (DMN) is current being best assessed for use. Validation of an HPLC methods an analytical technique for the measurement of 1, 4-dimehylnaphthalene with 2-methylnaphthalene as internal standard, required the assessment of the limit of detection (LOD) and limit of quantification (LOQ). This method will help determine the selection of good chromatographic conditions for the studying the behaviour of 1, 4-DMN in environmental samples. In this study, LOD and LOQ were measured using three different HPLC systems, two approaches were applied. The approaches are firstly by repeated injection (n=10) of a single low level standard solution (0.1 and 0.01 µg/ml) of 1, 4-DMN and 2-Methylnaphthalene into the three HPLC systems. The second approach was to determine the LOD and LOQ based upon the statistical data of the regression line of the calibration curve of 1,4-DMN and 2-Methylnaphthalene in the lower range concentrations0.02-0.1µg/ml. The approach of repeated injection of 0.01µg/ml was found to produce a lower LOD and LOQ compared to the calibration curve method by factors of 4 to 5. The diode array detector was less sensitive by a factor of approximately 4. The best system combining single wave length detector and autosampler injector gave values of LOD 0.001 µg/ml and LOQ 0.002 µg/ml for both 1, 4-DMN and 2-methylnaphthalene.

Presentation

Chemistery department / Faculty of Science -University of Zakho
2020-12
Coronavirus instructions for students and staffs

Similarities and differences between COVID-19 and influenza viruses Tips for COVID-19 prevention

 2020
Seminar's hall at chemistry department, Faculty of Science/ University of Zakho.
2017-12
Laboratory Safety

Oral presentation ontitled : General safety information and first aid

 2017
Faculty of Science, Department of Chemistry, University of Zakho
2017-05
انخفاض المستوى العلمي و التحصيل الدراسي لدى طلبة الجامعات

انخفاض المستوى العلمي و التحصيل الدراسي لدى طلبة الجامعات الاسباب والتوصيات

 2017
Faculty of Science, Department of Chemistry, University of Zakho
2014-02
Analysis Of Pesticide Residue By HPLC

Detemination the residue of pesticides, diffrent extraction methods and techniques.

 2014

Workshop

English Language Department/ Faculty of Humanities, University of Zakho, Kurdistan region-Iraq
2017-12
Teaching and Learning Approaches Workshop

Teaching and Learning Approaches Workshop

 2017
Seminar's hall at chemistry department, Faculty of Science/ University of Zakho.
2017-12
Workshop in Safety

health occupation and lab safety information

 2017
University of Zakho, Kurdistan region-Iraq.
2013-09
Workshop on e-Learning system design: Teacher-student websites

Participitant

 2013
Syngenta, Jealott’s Hill, Berks, UK.
2011-02
Interactions of pesticide application and formulation on residues in fruit and vegetables, sponsored by Association of Applied Biologists

participitated in poster presentation In UK, the potato industry needs to store large quantities of the potato. The major concern is sprouting, which is the inevitable cause for undesirable quality. Chlorpropham (CIPC) is a main... See more

participitated in poster presentation In UK, the potato industry needs to store large quantities of the potato. The major concern is sprouting, which is the inevitable cause for undesirable quality. Chlorpropham (CIPC) is a main sprout suppressant chemical applied as a hot fog to the stored potatoes. However, some residues of CIPC and possible presence of its main breakdown product 3-chloroaniline (3CA) can be found on the treated potatoes. For human risk assessment, there is a concern over 3CA. Recently, the European Communities Commission required the maximum residue level value of both 3CA and CIPC from 2011. Therefore, a consistent method is required to extract and estimate the residue of Chlorpropham and 3-Chloroaniline in potato samples. Methods for analysing CIPC are well documented but not for 3CA. In previous our work, a simplified method has been developed and validated to extract and analysis both CIPC and 3CA based on soaking overnight extraction coupled to HPLC - UV analysis using methanol as solvent extractant. The results of spiking potato skin showed a high recovery of CIPC (> 95%) but the recovery of its metabolite 3CA was less than 5 %. The reason of poor extraction can be due to the irreversible binding of 3CA with potato skin substrate. Therefore, to reduce that, suggestion was made to use a 50:50 mixture of methanol and 1M H2SO4. Experimental recoveries (n=5) at spiking level of 8 mg/kg were above 60% and 80% at ambient and 50 ºC temperature for 24 hours respectively. In order to optimize the extraction method, the final part of this study was to look at real potato samples taken from UK stores and treated with solid CIPC at two temperatures 450 ºC and 270 ºC at concentration levels 14 and 12 g/tonne respectively. The skin of each of these potatoes was extracted applying three different methanol-extracting solutions for 24 hours (100% methanol at lab temperature, 50% methanol in 1M H2SO4 at lab temperature and 50 ºC). The analysis results reported high residue levels of 3CA using hot temperature of mixture of methanol and sulfuric acid which was 2.5 mg/kg for potatoes treated at 450 ºC but lower than 0.2 mg/kg at 270 ºC. While the residue results of CIPC showed no exceed of the MRL level of CIPC (10 mg/kg).

 2011
Edinburgh, Scotland, UK.
2010-06
International potato processing and storage Convention

International potato processing and storage Convention

 2010
Edinburgh, Scotland/UK.
2008-05
Analytical science workshops of Waters Scottish New Technology Showcase (chromatographic technique)

Analytical science workshops of Waters Scottish New Technology Showcase (chromatographic technique)

 2008